Water quality. Determination of the toxicity of water samples on the embryo-larval development of the Japanese oyster (<i>Magallana gigas</i>) and the blue mussel (<i>Mytilus edulis or M. galloprovincialis</i>)

Water quality. Determination of the toxicity of water samples on the embryo-larval development of the Japanese oyster (Magallana gigas) and the blue mussel (Mytilus edulis or M. galloprovincialis)

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1 Scope

This document specifies a method for assessing the effects of chemical and aqueous samples on the embryo-larval development of marine bivalves. This method allows the determination of the concentration levels that result in an abnormality in embryo-larval development. This test is suitable for salinity ranges:

  • between 20 PSU (practical salinity unit) and 40 PSU for mussels, and

  • between 25 PSU and 35 PSU for oysters.

This method in this document applies to:

  • chemical substances and preparations,

  • marine and brackish waters,

  • streams and aqueous effluents (urban, agricultural, industrial effluents, etc.) as long as the salinity is adjusted or dilution is limited so that the aforementioned salinity ranges are respected,

  • aqueous extracts (pore water, elutriates, eluates and leachates) from sediments and petroleum products, and

  • samples of contaminated sediment or dredged material (see Annex C).