Water quality. Determination of the toxicity of water samples on the embryo-larval development of Japanese oyster (<i>Crassostrea</i> <i>gigas</i>) and mussel (<i>Mytilus</i> <i>edulis</i> or <i>Mytilus</i> <i>galloprovincialis</i>)

Water quality. Determination of the toxicity of water samples on the embryo-larval development of Japanese oyster (Crassostrea gigas) and mussel (Mytilus edulis or Mytilus galloprovincialis)

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1 Scope

This International Standard specifies a method for determining the effects of chemical and aqueous samples on the embryo-larval development of marine bivalves. It allows the determination of the concentration levels that result in an abnormality in embryo-larval development. This test is suitable for salinity ranges between 20 and 40 for mussels and between 25 and 35 for oysters. This method applies to

  • chemical substances and preparations,

  • marine and brackish waters,

  • streams and aqueous effluents (urban, agricultural, industrial effluents, etc.) as long as the salinity is adjusted and/or dilution is limited so that the aforementioned salinity ranges are respected, and

  • aqueous extracts (pore water, elutriates, eluates, and leachates) from sediments and petroleum products.