Water quality. Determination of the dissolved fraction of selected active pharmaceutical ingredients, transformation products and other organic substances in water and treated waste water. Method using high performance liquid chromatography and…

Water quality. Determination of the dissolved fraction of selected active pharmaceutical ingredients, transformation products and other organic substances in water and treated waste water. Method using high performance liquid chromatography and…

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1   Scope

This document specifies a method for the determination of the dissolved fraction of selected active pharmaceutical ingredients and transformation products, as well as other organic substances (see Table 1) in drinking water, ground water, surface water and treated waste water.
The lower application range of this method can vary depending on the sensitivity of the equipment used and the matrix of the sample. For most compounds to which this document applies, the range is ≥ 0,025 µg/l for drinking water, ground water and surface water, and ≥ 0,050 µg/l for treated waste water.
The method can be used to determine further organic substances or in other types of water (e.g. process water) provided that accuracy has been tested and verified for each case, and that storage conditions of both samples and reference solutions have been validated. Table 1 shows the substances for which a determination was tested in accordance with the method. Table E.1 provides examples of the determination of other organic substances.
Table 1Substances for which a determination was tested in accordance with this method
Common name
Chemical name (IUPAC a )
Molecular
formula
Molar
mass
CAS-RN b
    g/mol  
4-Acetylaminoantipyrine
N-(2,3-Dimethyl-5-oxo-1-phenyl-3-pyrazolin-4-yl)acetamide
C13H15N3O2 245,28 83-15-8
N4-Acetyl sulfamethoxazole
N-{4-[(5-Methyl-1,2-oxazol-3-yl)sulfamoyl]phenyl}-acetamide
C12H13N3O4S 295,32 21312-10-7
Diatrizoic acid (amidotricoic acid)
3,5-Bis(acetamido)-2,4,6-triiodobenzoic acid
C11H9I3N2O4 613,91 117-96-4
Atenolol
(RS)-2-[4-[2-Hydroxy-3-(1-methylethylamino) propoxy]phenyl]ethanamide
C14H22N2O3 266,34 29122-68-7
Bezafibrate
2-{4-[2-(4-Chlorbenzamido)ethyl]phenoxyl}-2-
methylpropanoic acid
C19H20ClNO4 361,80 41859-67-0
Bisoprolol
(RS)-1-[4-(2-Isopropoxyethoxymethyl)phenoxy]-3-
isopropylamino-2-propanol
C18H31NO4 325,45 66722-44-9
Carbamazepine
5H-Dibenzo[b,f]azepine-5-carbamide
C15H12N2O 236,27 298-46-4
Clarithromycin
(2R,3R,4S,5R,8R,9S,10S,11R,12R,14R)-11-[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy-5-ethyl-3,4-dihydroxy-9-[(2R,4R,5S,6S)-5-hydroxy-4-methoxy-4,6-dimethyl-oxan-2-yl]oxy-12-methoxy-2,4,8,10,12,14-hexa-
methyl-6-oxacyclotetradecane-1,7-dione
C38H69NO13 747,95 81103-11-9
Clofibric acid
2-(4-Chlorophenoxy)-2-methylpropanoic acid
C10H11ClO3 214,70 882-09-7
Dehydrato-Erythromycin (anhydro-erythromycin)
(2R,3R,4S,5S,8R,9S,10S,11R,12R)-11-{[4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-5-ethyl-3-hydroxy-9-[(5-hydroxy-4-methoxy-4,6-dimethyloxan-2-yl)oxy]-2,4,8,10,12,14-hexamethyl-6,15,16-trioxatricyclo[10.2.1.1{1,4}]hexadecane-7-one
C37H65NO12 715,91 23893-13-2
Diazepam
(RS)-7-Chlor-1-methyl-5-phenyl-1,3-dihydro-2H-1,4-
benzodiazepine-2-on
C16H13ClN2O 284,74 439-14-5
Diclofenac
2-[2-[(2,6-Dichlorphenyl)amino]phenyl]acetic acid
C14H11Cl2NO2 296,15 15307-86-5
10,11-Dihydro-10,11-dihydroxy carbamazepine
(5S,6S)-5,6-Dihydroxy-5,6-dihydrobenzo[b][1]benzazepie-11-carboxamide
C15H14N2O3 270,29 58955-93-4
Erythromycin
6-(4-Dimethylamino-3-hydroxy-6-methyl-oxan-2-yl)oxy-14-ethyl-7,12,13-trihydroxy-4-(5-hydroxy-4-methoxy-4,6-dimethyl-oxan-2-yl)-oxy-3,5,7,9,11,13-hexamethyl-1-oxacyclotetradecane-2,10-dione
C37H67NO13 733,93 114-07-8
4-Formylaminoantipyrine
N-(2,3-Dihydro-1,5-dimethyl-3-oxo-2-phenyl-1H-pyrazol-4-yl)formamide
C12H13N3O2 231,25 1672-58-8
Gemfibrozil
5-(2,5-Chlorophenoxy)-2,2-methylpropanoic acid
C15H22O3 250,34 25812-30-0
Ibuprofen
(RS)-2-[4-(2-Methylpropyl)phenyl]propanoic acid
C13H18O2 206,28 15687-27-1
Iomeprol
(±)-N,N′-Bis-(2,3-dihydroxypropyl)-5-[(2-hydroxy-acetyl)methylamino]-2,4,6-triiodo isophthalamide
C17H22I3N3O8 777,09 78649-41-9
Iopamidol
(S)-N,N′-Bis[2-hydroxy-1-(hydroxymethyl)ethyl]-5-[(2-hydroxypropanoyl)amino]-2,4,6-triiodobenzene-1,3-dicarbamide
C17H22I3N3O3 777,08 60166-93-0
Iopromide
(±)-N,N′-Bis(2,3-dihydroxypropyl)-2,4,6-triiodo-5-
(2-methoxyacetamido)-N-methylisophthalamide
C18H24I3N3O8 791,12 73334-07-3
Metoprolol
(RS)-1-(Isopropylamino)-3-[4-(2-methoxyethyl) phenoxy]propan-2-ol
C15H25NO3 267,36 37350-58-6
Naproxen
(S)-2-(6-Methoxy-2-naphthyl)propanoic acid
C14H14O3 230,26 22204-53-1
Oxazepam
(RS)-7-Chloro-3-hydroxy-5-phenyl-1,3-dihydro-2H-1,4-
benzodiazepin-2-on
C15H11ClN2O2 286,71 604-75-1
Phenazone
1,5-Dimethyl-2-phenyl-2,3-dihydro-1H-pyrazol-3-on
C11H12N2O 188,23 60-80-0
Primidone
5-Ethyl-5-phenylhexahydropyrimidin-4,6-dione
C12H14N2O2 218,25 125-33-7
Propyphenazone
1,5-Dimethyl-4-(1-methylethyl)-2-phenyl-1,2-dihydro-3H-
pyrazol-3-one
C14H18N2O 230,31 479-92-5
Roxithromycin
(3R,4S,5S,6R,7R,9R,11S,12R,13S,14R)-6-{[(2S,3R,4S,6R)-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy}-14-ethyl-7,12,13-trihydroxy-4-{[(2R,4R,5S,6S)-5-hydroxy-4-methoxy-4,6-dimethyloxan-2-yl]oxy}-3,5,7,9,11,13-hexamethyl-10-(2,4,7-trioxa-1-azaoctan-1-ylidene)-1-
oxacyclotetradecane-2-one
C41H76N2O15 837,05 80214-83-1
Sotalol
(RS)-4′-(1-Hydroxy-2-isopropylaminoethyl)
methanesulfonanilide
C12H20N2O3S 272,36 3930-20-9
Sulfamethoxazole
4-Amino-N-(5-methyl-1,2-oxazol-3-yl)benzene-sulfonamide
C10H11N3O3S 253,28 723-46-6
Temazepam
(RS)-7-Chloro-3-hydroxy-1-methyl-5-phenyl-1,3-dihydro-2H-1,4-benzodiazepin-2-one
C16H13ClN2O2 300,74 846-50-4
Trimethoprim
2,4-Diamino-5-(3,4,5-trimethoxybenzyl)pyrimidine
C14H18N4O3 290,32 738-70-5
a IUPAC: International Union of Pure and Applied Chemistry.
b CAS-RN: Chemical Abstracts System Registration Number.