STANDARD TEST METHOD FOR IN VITRO BIOLOGICAL ACTIVITY OF RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2 (RHBMP-2) USING THE W-20 MOUSE STROMAL CELL LINE

STANDARD TEST METHOD FOR IN VITRO BIOLOGICAL ACTIVITY OF RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2 (RHBMP-2) USING THE W-20 MOUSE STROMAL CELL LINE

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1.   Scope

This test method describes the method used and the calculation of results for the determination of the in-vitro biological activity of rhBMP-2 using the mouse stromal cell line W-20 clone 17 (W-20-17). This clone was derived from bone marrow stromal cells of the W++ mouse strain2.