Soil quality. Estimation of abundance of selected microbial gene sequences by quantitative polymerase chain reaction (qPCR) from DNA directly extracted from soil

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This document specifies the crucial steps of a quantitative polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract. The number of microbial gene sequences quantified by qPCR provides an estimation of the abundance of selected microbial groups in soil.