1 Scope
This document specifies a horizontal method for the detection of enteropathogenic
Vibrio species (spp.), which causes human illness in or via the intestinal tract. The species
detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.
It is applicable to the microbial plant biostimulants.
NOTE 1 The World Health Organization (WHO) has identified that V. parahaemolyticus, V. cholerae and V. vulnificus are the major contaminants of Vibrio spp. [2].
NOTE 2 For confirmation, it is possible to use PCR (Polymerase Chain Reaction) tests; in this case a validation is carried out by the laboratory for the procedure and data generated.
This document is applicable to the blends of fertilizing products where a blend is
a mix of at least two of the following component EU fertilising products categories:
Fertilizers, Liming Materials, Soil Improvers, Growing Media, Plant Biostimulants,
and where the following category Plant Biostimulants is the highest % in the blend
by mass or volume, or in the case of liquid form by dry mass. If Plant Biostimulants
is not the highest % in the blend, the European Standard for the highest % of the
blend applies. In case a blend of fertilizing products is composed of components in
equal quantity or in case the component EU fertilising products used for the blend
have identical formulations1, the user decides which standard to apply.

