What is BS EN 16418 - Cytotoxicity assessment of paper and board about?
BS EN 16418 is applicable to paper and board. BS EN 16418 is used for the determination of the cytotoxicity of aqueous extracts using a metabolically competent hepatoma cell line (HepG2).
BS EN 16418 specifies a test method for the laboratory assessment of the potential cytotoxic effect of paper and board intended to come into contact with foodstuffs using specifically the HepG2 cell line. Compared to the EN 15845, HepG2 cells are more representative of human oral exposure to xenobiotics, due to the presence in the cells of phase I, II and III enzymes of the metabolism.
Who is BS EN 16418 - Cytotoxicity assessment of paper and board for?
BS EN 16418 on cytotoxicity assessment of paper and board is useful for:
- The paper and board industry
- Paper mills
- Paper and paperboard manufacturing companies
- Suppliers and distributors for paper and board
- Laboratories for paper and board
- Paper analysis researchers
Why should you use BS EN 16418 - Cytotoxicity assessment of paper and board?
The food contact paper and board samples to be tested are extracted into the water. The extracts then undergo cytotoxicity assessment, and the results obtained are compared to the results of a non-cytotoxic control purified water (for which the rate of RNA synthesis is considered optimal and arbitrarily set at 100 %).
BS EN 16418 provides you with test method which is intended to evaluate the cytotoxic effect of water extracts from materials for wet foods intended for human consumption. The test evaluates the impact of the water extract on the rate of RNA (Ribonucleic Acid) synthesis by measuring the incorporation of a radioactive marker (tritiated uridine) in human cells (HepG2).
Following BS EN 16418 guidelines, help you for safety evaluation of food contact paper and board.