What is ISO/TS 21569-2 about?
ISO/TS 21569-2 is the second part of a multi-series that specifies a procedure for the detection of a DNA sequence present in a genetically modified linseed (Linum usitatissimum) line (event FP967, also named as “CDC Triffid”).
ISO/TS 21569-2 is applicable for the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs and seeds. The application of this method requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix for the purpose of analysis.
Who is ISO/TS 21569-2 for?
ISO/TS 21569-2 on the real-time PCR method for analysis on GMO’s is relevant to:
- GMO testing laboratories
- Biotechnologists
- Food technologists
- Food safety and regulatory bodies
- Certification bodies
Why should you use ISO/TS 21569-2?
Polymerase chain reaction (PCR) test qualifies and quantifies genetically modified organisms (GMO) present in food or feed samples.
Under ISO/TS 21569-2, DNA is extracted from the test sample applying a suitable method. The DNA analysis consists of two parts:
- Verification of the amount, quality and amplifiability of the extracted DNA, e.g. by means of a target tax on specific real-time PCR with primers amplifying a 68 bp long fragment from the linseed-specific (Linum usitatissimum) stearoyl-acyl carrier protein desaturase 2 gene (SAD)
- Detection of the Tnos-dfr construct in a real-time PCR
The method has been validated in a collaborative study coordinated by the German Federal Office of Consumer Protection and Food Safety (BVL), in accordance with the IUPAC protocol.
With the necessary guidance and compliance to ISO/TS 21569, you can explore the safety levels of GMO and derived products and consequently avoid cross-contamination risks.
What’s changed since the last update?
PD ISO/TS 21569-2:2021 supersedes PD ISO/TS 21569‑2:2012, which is withdrawn
The main changes compared with the previous edition are as follows:
- Single target copy integration into the genome has been updated
- An explanation of A/Spectinomycin resistance cassette juxtaposition has been added
- Minor typographical improvements have been made