Milk products. Enumeration of presumptive bifidobacteria. Colony count technique at 37 °C

Milk products. Enumeration of presumptive bifidobacteria. Colony count technique at 37 °C

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1   Scope

This International Standard specifies a method for the selective enumeration of presumptive bifidobacteria in milk products by using a colony count technique at 37 °C under anaerobic conditions.
The method is applicable to milk products such as fermented and non-fermented milks, milk powders, infant formulae, and starter cultures where these microorganisms are present and viable, and in combination with other lactic acid bacteria. (For proposed quality criteria of dairy products, see, for example, Codex Stan 243:2003 [6].)
Bifidobacteria used in milk products usually belong to the species (e.g. see References [7][8][16]):
  1. a) Bifidobacterium adolescentis;
  2. b) B. animalis subsp. animalis;
  3. c) B. animalis subsp. lactis;
  4. d) B. bifidum;
  5. e) B. breve;
  6. f) B. infantis;
  7. g) B. longum.