What is ISO 16649-3-Detection of β-glucuronidase positive Escherichia coli about?
ISO 16649 is an international standard on microbiology of the food chain that discusses a horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli. ISO 16649-3 provides the best industry guidance on detection and most probable number technique using 5-bromo-4- chloro-3-indolyl-ß-D-glucuronide Incorporating corrigendum to reduce the effect of Escherichia coli on human health. ISO 16649-3 is the third part of a multi-series that specifies a horizontal method for the detection and enumeration of β-glucuronidase positive Escherichia coli, through the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at (37 ± 1) °C, then at (44 ± 1) °C.
ISO 16649-3 applies to the:
- Products intended for human consumption and the feeding of animals
- Environmental samples in the area of food production and food handling
The method under ISO 16649-3 is suitable for the enumeration of cells of E. coli that might have been subjected to stress arising from dehydration, freezing, and exposure to saline (such as marine) environment or damaged by disinfectants such as chlorine-containing products.
Note 1: The method under ISO 16649-3 has not been fully evaluated for all matrices (e.g. for milk and milk products).
Note 2: A limitation of the applicability of ISO 16649-3 is imposed by the susceptibility of the method to a large degree of variability.
Who is ISO 16649-3-Detection of β-glucuronidase positive Escherichia coli for?
ISO 16649-3 on the detection of β-glucuronidase positive Escherichia coli is relevant to:
- Food scientists
- Food technologists
- Food laboratories
- Food health and safety departments
- Microbiologists
Why should you use ISO 16649-3-Detection of β-glucuronidase positive Escherichia coli?
β-glucuronidase positive Escherichia coli are strains of E. coli which, at 44 °C, form typical blue or blue-green colonies on tryptone bile glucuronide medium (TBX) under the conditions specified in the procedure. ISO 16649-3 provides you with the detection method, enumeration method, performance testing for the quality assurance of the culture media, apparatus and glassware, sampling, procedures, and much more.
The methods and procedures provided by ISO 16649-3 assist in detecting and enumerating of beta-glucuronidase-positive Escherichia coli, improve food quality and ensure reduction of health issues due to consumption of β-glucuronidase positive Escherichia coli.
With obedience and compliance to ISO 16649-3, you can enhance the efficacy of the food products and manufacture a standardized high-quality product.
What is changed since the last update?
ISO 16649-3:2015 supersedes ISO/TS 16649-3:2005, which is withdrawn. ISO 16649-3:2015 has made some editorial changes compared to the previous edition ISO/TS 16649-3:2005. These include:
- In 4.1.4, the first line, “(22 ± 2) h” has been replaced with “(21 ± 3) h”
- In 4.2.5, the first line, “(22 ± 2) h” has been replaced with “(21 ± 3) h”
- Table 1 and Table 2 has been replaced
- In 9.1.4, the first line, “(22 ± 2) h” has been replaced with “(21 ± 3) h”
- In 9.2.5, the second line, “three” has been replaced with “six”