Infant formula and adult nutritionals. Determination of β-carotene, lycopene and lutein by reversed-phase ultra-high performance liquid chromatography (RP-UHPLC)

Infant formula and adult nutritionals. Determination of β-carotene, lycopene and lutein by reversed-phase ultra-high performance liquid chromatography (RP-UHPLC)

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What is ISO 23443about?  

ISO 23443 specifies a method for the quantitative determination of β-carotene and lycopene in infant formula and adult nutritionals in solid (i.e. powders) or liquid (i.e. ready-to-feed liquids and liquid concentrates) forms using reversed-phase ultra-high performance liquid chromatography (RP-UHPLC) and UV-visible detection. 

The application range runs from 1 μg/100 g to 1 500 μg/100 g for lycopene and from 1 μg/100 g to 2 250 μg/100 g for β-carotene. 

Note: The method does not apply to materials that contain measurable levels of β-apo-8′-carotenal. 

Who is ISO 23443 for?  

ISO 23443 on infant formula and adult nutritionals is relevant to: 

  • Manufacturers of infant formula and adult nutritionals 
  • Nutrition testing and certification bodies 
  • Food technologists 
  • Regulatory bodies 

Why should you use ISO 23443?  

Infant formulas are foods designed to feed babies and infants less than 12 months of age while adult nutrition refers to products used as supplement for improved health.  

Lutein, β-carotene and lycopene are among the carotenoids present in human milk and are added to infant formula and adult nutritionals. Lutein may play a role in vision and cognitive function, and β-carotene has provitamin A activity. Accurate and precise measurements of these added ingredients are important for ensuring their presence in the allowable ranges. 

ISO 23443 provides you with the necessary testing methods to ensure the right ingredients with the right nutritional content and value is provided as per the need of the specific product.  

As a result, standardised methods to assess the nutritional value in compliance with ISO 23443, helps foster reliability and trust for your products, by keeping in mind the health requirements of your consumers and meeting food safety and regulatory norms.