Foodstuffs. HPLC method for the determination of xanthophylls in fish flesh - Identification of the enantiomer ratio of astaxanthin

Foodstuffs. HPLC method for the determination of xanthophylls in fish flesh - Identification of the enantiomer ratio of astaxanthin

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What is DD CEN/TS 16233-2-Identification of the enantiomer ratio of astaxanthin in fish flesh about?  

DD CEN/TS 16233 is a technical specification on foodstuffs that discusses the HPLC method for the determination of xanthophylls in fish flesh to ensure that the fish flesh is safe for consumption.  

DD CEN/TS 16233-2 is the second part of the DD CEN/TS 16233 series that describes a method for the determination of the astaxanthin enantiomer ratio in fish flesh by high-performance liquid chromatography (HPLC).  

The racemic astaxanthin mixtures have been observed in the shrimp species Penaeus and in some other orders of higher crustaceans. Therefore, fish exclusively fed with shrimp meal could also contain racemic astaxanthin and could erroneously be regarded as fish fed with synthetic astaxanthin. 

Who is DD CEN/TS 16233-2-Identification of the enantiomer ratio of astaxanthin in fish flesh for? 

DD CEN/TS 16233-2 on the identification of the enantiomer ratio of astaxanthin in fish flesh is useful for: 

  • Food scientists 
  • Fish flesh manufacturers and suppliers 
  • Food technologists  
  • Food laboratories  
  • Food health and safety departments 

Why should you use DD CEN/TS 16233-2-Identification of the enantiomer ratio of astaxanthin in fish flesh 

Three stereoisomers of all-E-astaxanthin (two enantiomers and a meso-form) exist, named (3R,3’R)-, (3S,3’S)- and (3R,3’S, meso)-all-E-astaxanthin. These isomers can be determined by chromatography on a chiral HPLC column. Salmonids receive their astaxanthin through the diet and the flesh isomer composition reflects the dietary source of astaxanthin.  

Astaxanthin is a red fat-soluble pigment that belongs to the family of the xanthophylls; the oxygenated derivatives of carotenoids and is closely related to β-carotene, lutein, and zeaxanthin [3]. Astaxanthin is a component of the pigments included in crustacean, salmonids, and other farmed fish feeds. 

DD CEN/TS 16233-2 provides you with guidance on apparatus, sample preparation and extraction, large scale extraction, small scale extraction, HPLC identification, HPLC conditions, test report, etc. 

The HPLC test method provided by DD CEN/TS 16233-2 assists you in the determination of xanthophylls in fish flesh and identification of the enantiomer ratio of astaxanthin and ensure reduction in the risk to human health due to consumption of high astaxanthin content. 

With obedience and compliance to DD CEN/TS 16233-2, you can enhance efficacy in the production of fish flesh, enable quality mass production and produce a standardized high-quality product.