1 Scope
This European Standard specifies a method for the determination of vitamin E in foods
by high performance liquid chromatography (HPLC). The determination of vitamin E content
is carried out by measurement of α-, β-, γ- and δ-tocopherol. This method has been
validated in two interlaboratory studies. The first study was for the analysis of
α-tocopherol in margarine and milk powder ranging from 9,89 mg/100 g to 24,09 mg/100 g.
The second study was for the analysis of α-, β-, γ- and δ-tocopherol in milk powder
and of α-, and β- tocopherol in oat powder ranging from 0,057 mg/100 g (β-tocopherol)
to 10,2 mg/100 g (α-tocopherol).
NOTE The vitamin E activity can be calculated from the tocopherol content assuming appropriate factors as given in [1], [2], [3] and [4].