This Technical Specification specifies a method for the determination of fumonisin B₁ (FB₁) and fumonisin B₂ (FB₂) in processed maize-containing foods for infants and young children by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection (FLD). This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples ranging from 112 µg/kg to 458 µg/kg for FB₁+FB₂, 89 µg/kg to 384 µg/kg for FB₁ and 22 µg/kg to 74 µg/kg for FB₂.

For further information on the validation see Clause 8 and Annex B.