Food authenticity. Determination of 16-O-Methylcafestol content of green and roasted coffee. HPLC-method

Food authenticity. Determination of 16-O-Methylcafestol content of green and roasted coffee. HPLC-method

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1 Scope

This document specifies a high-performance liquid chromatography (HPLC) method for determining the 16-O-Methylcafestol content in green and roasted coffee.

The method is suitable for a content of 40 mg/kg to 1 600 mg/kg of 16-O-Methylcafestol of green and roasted coffee, respectively. The collaborative study has shown that mass fractions also between 20 mg/kg to 40 mg/kg can be successfully analysed depending on the laboratory equipment.

The compliance assessment process is not part of this document.