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Fine bubble technology. Evaluation method for determining the reactive oxygen species in ultrafine bubble dispersions - APF (3'-(p-aminophenyl) fluorescein) assay

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1 Scope

This document describes the procedure for testing and evaluating the generation of ROS such as OH, O2·−, H2O2, and O3 in MB and UFB dispersions using the APF assay.

This method is suited for systems generating ROS at physiological levels, not in large quantities, and complements the probe kinetic model for detecting H2O2 in MB and UFB dispersions.