Fertilizers. Determination of N-(n-Butyl)thiophosphoric acid triamide (NBPT) and N-(n-Propyl)thiophosphoric acid triamide (NPPT). Method using high-performance liquid chromatography (HPLC)

Fertilizers. Determination of N-(n-Butyl)thiophosphoric acid triamide (NBPT) and N-(n-Propyl)thiophosphoric acid triamide (NPPT). Method using high-performance liquid chromatography (HPLC)

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What is BS EN 16651-NBPT and NPPT in fertilizers about? 

BS EN 16651 is a standard that serves technical specifications for determining NBPT and NPPT in fertilizers, ensuring fertilizer use efficiency and protection against ammonia volatilization in agricultural applications. 

BS EN 16651 specifies the method for the quantitative determination of the urease inhibitors N-(n-Butyl) thiophosphoric acid triamide (NBPT, CAS-No. 94317-64-3) and N-(n-Propyl) thiophosphoric acid triamide (NPPT, CAS-No. 916809-14-8) content in urea-based fertilizers using high-performance liquid chromatography (HPLC). 

Who is BS EN 16651-NBPT and NPPT in fertilizers for? 

BS EN 16651 on NBPT and NPPT in fertilizers applies to: 

  • Manufacturers of fertilizers 
  • Dealers and importers of fertilizers 
  • Testing labs for fertilizers 
  • Safety assurance services 
  • Regulatory bodies 

Why should you use BS EN 16651-NBPT and NPPT in fertilizers? 

NBPT inhibits the activity of the urease enzyme. Urease is found in soil microorganisms in large quantities. It transforms urea into ammonia, which is prone to volatilization if produced quicker than plants can use it. 

BS EN 16651 provides users guidance on principle, reagents, sampling, procedure, precision, and test report, ensuring the accurate detection of NBPT and NPPT in fertilizers. 

BS EN 16651 provides users guidance on the high-performance liquid chromatography (HPLC) method. BS EN 16651 also provides users with a step-by-step procedure for the method, enabling users to do the technique correctly.  

The adoption of BS EN 16651 allows users to determine NBPT and NPPT content in fertilizers, ensuring the agronomic efficiency as a urease inhibitor for improved fertilizer use efficiency in agricultural applications.