What is ISO 10315 about?
ISO 10315 specifies a method for the gas-chromatographic determination of nicotine in total particulate matter from the mainstream smoke. ISO 10315 includes principles, reagents, apparatus, procedures, repeatability and reproducibility as well as alternative gas-chromatographic procedures and analysis precautions for determining nicotine from cigarette smoke
Note 1: ISO 20778 and ISO 22253 provide the determination method of nicotine in smoke with an intense smoking regime.
Note 2: The smoking of cigarettes and the collection of mainstream smoke are carried out according to ISO 4387.
Who is ISO 10315 for?
ISO 10315 on gas-chromatographic method for cigarettes is useful for:
- Pharmaceutical industries
- Medical and forensic departments
- Environmental monitoring laboratories
- Natural water bodies
- Researchers
Why should you use ISO 10315?
Tobacco smoke compounds have been investigated extensively because of the researchers’ organoleptic or biological interest. It was observed that around 2500 compounds are found and known as tobacco smoke components.
A methodology known as gas chromatography has been developed and practiced, to determine these high quantity elements from tobacco smoke.
ISO 10315provides you with a method for the gas-chromatographic that describes the determination of total and nicotine-free dry particulate matter (NFDPM) in total particulate matter from the main-stream smoke to separate the compounds that has properties like high volatility, low molecular weights, thermal stability etc. It helps you to compare the concentration of these cigarette smoke gas-phase constituents among individual puffs.
What’s changed since the last update?
BS ISO 10315:2021 supersedes BS ISO 10315:2013, which is withdrawn.
The main changes compared to the previous edition are as follows:
- The term "smoke condensate(s)" has been replaced with either “total particulate matter” or "total particulate matter from the mainstream smoke" throughout the document.
- Extraction solution and calibration solutions stored at low temperature, are equilibrated to ambient temperature before use (5.7).
- The linear regression equation for calibration is calculated by regression analysis with the area ratios in accordance with the nicotine concentrations (7.3).
- Data in Clause 9 have been replaced with the results of ISO/TR 19478‑1.

