Cigarettes. Determination of benzo[a]pyrene in cigarette mainstream smoke using GC/MS - Method using cyclohexane as extraction solvent

What is ISO 22634‑2 - Determination of benzo[a]pyrene in cigarette smoke about ? 

ISO 22634‑2 is a standard that covers methods for the determination of benzo[a]pyrene in cigarette mainstream smoke that enables limiting the presence of benzo[a]pyrene in cigarettes to ensure the safety of users. 

ISO 22634‑2 specifies a method for the determination of benzo[a]pyrene (B[a]P) in the total particulate matter (TPM) of cigarette mainstream smoke using gas chromatography/mass spectrometry (GC/MS) with cyclohexane as extraction solvent. 

ISO 22634‑2 method is specified using ISO 3308 smoking parameters. ISO 22634‑2 document provides an alternative method to that specified in ISO 22634-1, with a different clean-up, and a shorter total analytical run allowing a potential increase of sample throughput in comparison with ISO 22634-1. 

Who is ISO 22634‑2 - Determination of benzo[a]pyrene in cigarette smoke for ? 

ISO 22634‑2 on determination of benzo[a]pyrene in cigarette smoke is applicable to: 

• Manufacturers of cigarettes 
• Dealers and importers of cigarettes 
• Laboratories for cigarettes 
• Regulatory bodies 

Why should you use ISO 22634‑2 - Determination of benzo[a]pyrene in cigarette smoke ? 

Tobacco smoke carcinogens are cofactors that work together with the human papillomavirus (HPV) to raise the risk of cervical cancer progression. BaP, a significant carcinogen found in cigarette smoke, has been found in cervical mucus and may interact with HPV. 

ISO 22634‑2 provides you guidance on alternative method on gas chromatography/mass spectrometry (GC/MS) with cyclohexane as extraction solvent which is a result of collaborative experiment in which many countries participated that ensures the validity and authenticity of the method. ISO 22634‑2 also provides you with a step-by-step procedure for the method which enables you to do the method correctly. 

Guidance in ISO 22634‑2 allows you to detect the benzo[a]pyrene in cigarette levels in cigarettes and enables you to limit their presence according to regulatory requirements. 

What’s changed since the last update ? 

BS ISO 22634‑2:2019 supersedes BS ISO 22634‑2:2017, which is now withdrawn. 

Changes in BS ISO 22634‑2:2019 include - 

• Reagents have modified by adding nitrogen 
• Storage of standard solutions has been modified 
• Sample clean-up has been modified 
• Vortex mixer has been added 
• Bibliography has been extended