What is ISO 20397-2 about?
ISO 20397-2 is one of a series of documents for Biotechnology and specifies general requirements and recommendations for quality assessments and control of massively parallel sequencing (MPS) data. It covers post raw data generation procedures, sequencing alignments, and variant calling.
ISO 20397-2 also gives general guidelines for the validation and documentation of MPS data. ISO 20397-2 does not apply to any processes related to de novo assembly.
Who is ISO 20397-2 for?
ISO 20397-2 on biotechnology is useful for-
- Manufacturers of Biotechnologies
- Services provider for Massively parallel sequencing in biotechnology.
- Biomedical laboratories and centres of biotechnologies
- Engineers and developers of biotechnologies
Why should you use ISO 20397-2?
Massively parallel sequencing (MPS) is a high-throughput analytical approach to nucleic acid sequencing utilizing massively parallel processing, that allows whole genomes, transcriptomes, and specific nucleic acid targets from different organisms to be investigated in a relatively short time.
ISO 20397-2 guidelines outlines general requirements and recommendations for quality assessments and control of MPS which is used in many life science disciplines permitting determination and high throughput analysis of millions and thousands of millions of nucleotide bases.
ISO 20397-2 also provides knowledge of data quality, Quality control for nucleic acid sequencing data handling and list of considerations for quality evaluation of MPS sequencing data, and the specific recommendations for different MPS platforms that ensure high-quality evaluation of sequencing data and reliability to trust.