What is BS EN 17270-Determination of theobromine in animal feeding stuffs about?
BS EN 17270 is a standard that covers the determination of theobromine in animal feeding stuffs that includes cocoa derived ingredients that enable limiting of the contamination and toxicity in animal feeds.
BS EN 17270 specifies a test method for the determination of theobromine in feed material or compound feed in the working range 27 mg/kg to 307 mg/kg using liquid chromatography coupled to a UV detector (HPLC-UV) or in the working range 49 mg/kg to 307 mg/kg using liquid chromatography with tandem mass spectrometry (LC-MS/MS).
BS EN 17270 method has been fully validated using complementary compound feed for adult dogs and complementary compound feed for horses.
BS EN 17270 method is also considered applicable for determining theobromine in baking chocolate using either HPLC-UV or LC-MS/MS systems. The working range can be extended provided the extended range is validated.
Who is BS EN 17270-Determination of theobromine in animal feeding stuffs for?
BS EN 17270 on determination of theobromine in animal feeding stuffs applies to:
- Manufacturers of animal feed
- Dealers and importers of animal feed
- Testing houses and labs for animal feed
Why should you use BS EN 17270-Determination of theobromine in animal feeding stuffs?
Theobromine is naturally present in the cacao tree and its seeds and consequently in cocoa products and by-products. Cocoa bean shells, cocoa bean meal, cocoa germs and discarded confectionery are used for feed purposes in Europe. Maximum levels of theobromine in feeding stuffs are controlled by EU regulations.
BS EN 17270 provides you guidance on principle, reagents, apparatus, procedure, chromatographic analysis, calculation of results, accuracy and test report which ensures the safety and protection of animal feeds.
BS EN 17270 provides you guidance on the liquid chromatography method to determine the content of Theobromine in animal feeds. BS EN 17270 also ensures you perform the method accurately by providing you with the step-by-step procedure for the method.
The guidance in BS EN 17270 enables you to identify the content of Theobromine in animal feeds and enables you to limit its presence according to regulatory requirements.