Animal feeding stuffs: Methods of sampling and analysis. Determination of Deoxynivalenol, Aflatoxin B1, Fumonisin B1 & B2, T-2 & HT-2 toxins, Zearalenone and Ochratoxin A in feed materials and compound feed by LC-MS/MS

Animal feeding stuffs: Methods of sampling and analysis. Determination of Deoxynivalenol, Aflatoxin B1, Fumonisin B1 & B2, T-2 & HT-2 toxins, Zearalenone and Ochratoxin A in feed materials and compound feed by LC-MS/MS

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1   Scope

This document's method of analysis is applicable for the determination of:
  • — deoxynivalenol (DON) in the tested range of 100 µg/kg to 3 300 µg/kg,
  • — aflatoxin B1 (AfB1) in the tested range of 2,5 µg/kg to 440 µg/kg,
  • — fumonisin B1 (FB1) in the tested range of 690 µg/kg to 7 500 µg/kg,
  • — fumonisin B2 (FB2) in the tested range of 200 µg/kg to 2 500 µg/kg,
  • — T-2 toxin in the tested range of 7,5 µg/kg to 360 µg/kg,
  • — HT-2 toxin in the tested range of 14 µg/kg to 1 800 µg/kg,
  • — zearalenone (ZEN) in the tested range of 30 µg/kg to 600 µg/kg, and
  • — ochratoxin A (OTA) in the tested range of 10 µg/kg to 230 µg/kg
in cereals and cereal-based compound feed by liquid-chromatography tandem mass spectrometry (LC-MS/MS). The actual working ranges could extend beyond the tested ranges.