Animal feeding stuffs: Methods of sampling and analysis. Detection and enumeration of Pediococcus spp. used as feed additive

Animal feeding stuffs: Methods of sampling and analysis. Detection and enumeration of Pediococcus spp. used as feed additive

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1   Scope

This document specifies general rules for the enumeration of pediococci in feeding stuffs (additives, premixtures and compound feeds excluding mineral feeds) that contain pediococci as a single microorganism component or in a mixture with other microorganisms. Applying the method to premixtures and compound feeds with critical amounts of copper demands a special procedure (see A.2). The document is not applicable to mineral feeds which are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40 % crude ash (Regulation (EC) 767/2009) [3]. There are different categories of feed samples:
  1. a) Additives containing about 1010 colony forming units (CFU)/g;
  2. b) Premixtures containing about 1011 CFU/kg;
  3. c) Compound feeds, meal or pellets which contain about 109 CFU/kg.