Animal feeding stuffs. Isolation and enumeration of yeast probiotic strains

Animal feeding stuffs. Isolation and enumeration of yeast probiotic strains

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1   Scope

This European Standard defines general rules for the enumeration of probiotic yeasts in feed samples (additives, premixtures and feeding stuffs) that contain yeast as a single microorganism component or in a mixture with other microorganisms. The standard is not applicable to mineral feeds which are defined as complementary feedingstuffs composed mainly of minerals and containing at least 40% crude ash (Council Directive 79/373/EEC) [4].
There are different categories of feed samples:
  1. a) Additives which contain about 109 CFU/g to 1010 CFU/g (CFU = colony forming units):
  2. b) Premixtures which contain about 108 CFU/g,
  3. c) Feeds, meal or pellets, which contain about 106 CFU/g and include complete feedingstuffs, and milk replacers.
The detection limit is as defined in EN ISO 7218.