Animal feeding stuffs. Determination of decoquinate by HPLC with fluorescence detection

Animal feeding stuffs. Determination of decoquinate by HPLC with fluorescence detection

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What is BS EN 16162 - Decoquinate in animal feeds about?

BS EN 16162 is a standard that serves technical specifications for determining decoquinate in animal feeds, enabling the prevention of coccidiosis in animals.

BS EN 16162 specifies a method for the determination of decoquinate. This high-performance liquid chromatographic (HPLC) method with fluorescence detection applies to the quantification of decoquinate content in complete and complementary compound feeds, medicated feeds, semi-liquid feeds, premixtures and feed additives.

BS EN 16162 method was fully validated from LOQ to 60 000 mg/kg on different matrices during an international collaborative study, especially on complete compound feeds for poultry, at a trace contamination level of 3 mg/kg and European authorized level of 20 mg/kg to 40 mg/kg.

In BS EN 16162 method, the limit of detection is between 0.1 mg/kg and 0.3 mg/kg and the limit of quantification is around 0.5 mg/kg. These limits were validated during the collaborative study, from results on the blank feed. Lower limits of detection or quantification could be reached but a single laboratory validation is then requested.

Who is BS EN 16162 - Decoquinate in animal feeds for?

BS EN 16162 on the determination of decoquinate in animal feeds applies to:

  • Manufacturers of animal feeds
  • Dealers and importers of animal feeds
  • Testing labs for animal feeds
  • Safety assurance services
  • Regulatory bodies

Why should you use BS EN 16162 - Decoquinate in animal feeds?

Decoquinate in animal feeds is a feed additive. A feed additive is a nutrient or medication supplement for cattle. Decoquinate is used for the prevention of coccidiosis in animals. It is also used to promote growth and fattening in chickens.

BS EN 16162 provides users guidance on a test method for the determination of decoquinate by HPLC with fluorescence detection method. BS EN 16162 also provides users with a step-by-step procedure for the method, enabling users to do the method correctly.

BS EN 16162 provides users guidance on principle, reagents, sampling, sample preparation, procedure, calculations, precision, and test report, enabling the accurate detection of decoquinate in animal feeds.

With obedience and compliance to BS EN 16162, users can develop and assess animal feeding stuffs to determine the content of decoquinate, enabling growth promotion and prevention of coccidiosis in animals.