Algae and algae products. Determination of the uronic acids content of brown seaweed and alginate products

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This document specifies a method for the quantitative determination of total uronic acids by High Performance Anion Exchange Chromatography coupled with Pulsed Amperometric Detection (HPAEC-PAD) after acid hydrolysis of the samples for algae and algae products. It specifies a method for the determination in one single analysis of mannuronic, glucuronic and guluronic acids in brown seaweed, and mannuronic and guluronic acids in alginate products. The sum of the individual uronic acid values is used for determining the total uronic acid content.